Born in Edinburgh, Jo Milne graduated in 1989 from Edinburgh College of Art and Edinburgh University with an MA in Fine Art. She was later awarded an MA in Printmaking from Camberwell College of Art and a Ph.D. in Fine Art from the University of Barcelona. She currently lives and works between Edinburgh, Barcelona, and La Segarra.

Her work has received numerous awards, for example from the Elizabeth Greenshields Foundation (Canada) and the Arena Foundation (Barcelona) and she has been an artist in residence at the Irish Museum of Modern Art (Dublin) and the Museu d’art de Sabadell (Sabadell). Her work has been exhibited extensively, with solo shows at Espai Volart, Fundació Vila Casas, (Barcelona), Cultural la Mercè, (Girona), La Sala (Vilanova i la Geltru), Grande Fontaine (Switzerland) and Amber Roome (Edinburgh). Other exhibitions include Galeria Trama (Barcelona), The Talbot Rice Gallery (Edinburgh), Herbert Read Gallery (Canterbury), Sarah Myerscough (London) and Hannah McClure Centre (Dundee).
Collections include Edinburgh College of Art, The University of Edinburgh, Museu de Sabadell, La Caixa and the Ayuntamiento de Pamplona (Spain).